Characters
- Simoun - Crisostomo Ibarra in disguise, left for dead at the end of Noli Me Tangere, has resurfaced as the wealthy jeweler, Simoun, sporting a beard, blue-tinted glasses, and a revolver. Fueled by his mistreatment at the hands of the Spaniards and his fury at Maria Clara's fate.
- Basilio - Son of Sisa. A graduating Medical Student who befriended Simoun. This is the same Basilio introduced in Noli Me Tangere.
- Isagani Villamor - Basilio's friend, ex-beau of Paulita Gomez and the man who removed the explosive lamp from the Captain Tiago's house, thus sabotaging Simoun's plans.
- Kabesang Tales - Cabeza Telesforo Juan de Dios, a former cabeza de barangay (barangay head) of Sagpang, a barangay in San Diego's neighboring town Tiani, who resurfaced as the feared Luzón bandit Matanglawin
- Don Custodio - Custodio de Salazar y Sánchez de Monteredondo, a famous "journalist" who was asked by the students about his decision for the Academia de Castellano. In reality, he is quite an ordinary fellow who married a rich woman in order to be a member of Manila's high society.
- Paulita Gomez - The girlfriend of Isagani and the niece of Doña Victorina, the old Indio who passes herself off as a Peninsular, who is the wife of the quack doctor Tiburcio de Espadaña. In the end, she and Juanito Peláez are wed, and she dumps Isagani, believing that she will have no future if she marries him.
- Macaraig - One of Isagani's classmates at the University of Santo Tomas. He is a rich student and serves as the leader of the students yearning to build the Academia de Castellano.
- Father Florentino - Isagani's godfather, and a secular priest; was engaged to be married, but chose to be a priest after being pressured by his mother, the story hinting at the ambivalence of his decision as he chooses an assignment to a remote place, living in solitude near the sea.
- Juli San Jose - Juliana de Dios, the girlfriend of Basilio, and the youngest daughter of Kabesang Tales. To claim her father from the bandits, she had to work as a maid under the supervision of Hermana Penchang. Eventually, she was freed but committed suicide after Father Camorra attempted to rape her.
- Juanito Pelaez - A favorite student of the professors. They belong to the noble Spanish ancestry.
- Doña Victorina - Victorina delos Reyes de Espadaña, known in Noli Me Tangere as Tiburcio de Espadaña's cruel wife. She is the aunt of Paulita Gomez, and favors Juanito Pelaez than Isagani. Although of Indio ideology, she considers herself as one of the Peninsular.
- Father Camorra - The lustful parish priest of Tiani, San Diego's adjacent town who has longtime desires for young women. He nearly raped Juli causing the latter to commit suicide.
- Ben-Zayb - The pseudonym of Abraham Ibañez, a journalist who believes he is the "only" one thinking in the Philippines. Ben-Zayb is an anagram of Ybanez, an alternate spelling of his name.
- Placido Penitente - A student of the University of Santo Tomas who was very intelligent and wise but did not want, if not only by his mother's plea, to pursue his studies. He also controls his temper against Padre Millon, his physics teacher.
- Hermana Penchang - Sagpang's rich pusakal (gambler). She offers Huli to be her maid so the latter can obtain money to free Kabesang Tales. Disbelieving of Huli and her close friends, she considers herself as an ally of the friars.
- Tiburcio de Espadaña - Don Tiburcio is Victorina de Espadaña's lame husband. He is currently on hiding with Father Florentino.
- Father Írene - Captain Tiago's spiritual adviser. Although reluctant, he helped the students to establish the Academia de Castellano after being convinced by giving him a chestnut. The only witness to Captain Tiago's death, he forged the last will and testament of the latter so Basilio will obtain nothing from the inheritance.
- Quiroga - A Chinese businessman who dreamed of being a consul for his country in the Philippines. He hid Simoun's weapons inside his house.
- Don Timoteo Pelaez - Juanito's father. He is a rich businessmen and arranges a wedding for his son and Paulita. He and Simoun became business partners.
- Tandang Selo - Father of Kabesang Tales. He raised the sick and young Basilio after he left their house in Noli me Tangere. He died in an encounter on the mountains with his son Tales.
- Father Fernández - The priest-friend of Isagani. He promised to Isagani that he and the other priests will give in to the students' demands.
- Sandoval - The vice-leader of Macaraig's gang. A Spanish classmate of Isagani, he coerces his classmates to lead alongside him the opening of the Spanish language academy.
- Hermana Báli - Another gambler in Tiani. She became Huli's mother-figure and counselor; helped to release Kabesang Tales from the hands of bandits.
- Father Millon - The Physics teacher of the University of Santo Tomas. He always becomes vindictive with Placido and always taunts him during class.
- Tadeo - Macaraig's classmate. He, along with the other three members of their gang, supposedly posted the posters that "thanked" Don Custodio and Father Irene for the opening of the Academia de Castellano.
- Leeds - An American who holds stage plays starring decapitated heads; he is good friends with Simoun.
- Tano - Kabesang Tales's elder son after his older sister, Lucia died in childhood. He is currently one of the Guardia Civil. He then returned under the name Carolino after his exile in Caroline Islands.
- Pepay - Don Custodio's supposed "girlfriend". A dancer, she is always agitated of her "boyfriend"'s plans. She seems to be a close friend of Macaraig.
- Gobernador General - The highest-ranking official in the Philippines during the Spanish colonial period, this unnamed character pretends that what he is doing is for the good of the Indios, the local citizens of the country, but in reality, he prioritizes the needs of his fellow Spaniards living in the country.
- Father Hernando de la Sibyla - A Dominican friar introduced in Noli Me Tangere, now the vice-rector of the University of Santo Tomas
- Pecson-classmate who had no idea on the happenings occurring around him. He suggested that they held the mock celebration at the panciteria. .
- Father Bernardo Salvi- Former parish priest of San Diego, now the director and chaplain of the Santa Clara convent.
- Captain Tiago - Santiago delos Santos, although making a cameo appearance, Captain Tiago is Maria Clara's stepfather and the foster-father to Basilio. His health disintegrates gradually because of the opium he was forced to smoke given to him by Father Irene.
